Wheat Transformation with ScTPS1-TPS2 Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress

Wheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used obtain transgenic wheat plants increase trehalose synthesis. Mature embryos were transformed using pGreen rd29A::TPS1-TPS2 or 35S::TPS1-TPS2 constructs. The transgene presence mature leaves of T3 confirmed by sequencing PCR fragment inserted transgene. Transgenic and NT submitted stress for eight days. Transformed lines retained higher relative water content than during stress, Rubisco activity unaffected. Plants with 35S construct showed lower photosynthetic rate fructose 1–6-bisphosphatase (FBPase) drought, suggesting that constitutive sucrose caused reduced ribulose 1,5-bisphosphate (RuBP) regeneration. Lines rd29A promoter after days as RuBP regeneration had biomass grain weight drought. These results suggest improves photosynthesis induces changes some Calvin-cycle enzymes, reflected plant metabolism growth.